Shoot regeneration from callus: After three weeks of culture, in order to propagation of competent callus masses, the white and friable calluses (regardless the explant type) were collected and sub-cultured in MS medium CIM9 for extra 2 weeks in dark place, then samples were transferred to photoperiod condition with 16:8 h lightness-darkness. Figure 7 shows the first presage of shoot initiation. Regenerated shoots can be transferred for rooting and soil acclimatization.
For root formation the amount of auxins have significant effect on rooting of shoots obtained from callus and 2 mg L-1 NAA in MS medium have the best result. This experiment was just focused on callus formation from different explant type and the effect of various hormone concentrations on callus initiation for shoot regeneration from competent callus as well.