The optimal media and growth conditions of an edible wild mushroom,
Phlebopus portentosus strain CMUHH121-005, were investigated for inoculum
production. The optimum temperature for mycelial growth was 30ºC and the optimum pH
was 4.0. Malt extract and yeast extract as carbon and nitrogen sources respectively, with a
C:N ratio of 10:1, were most suitable. The biomass production in 16 types of cereal grain
media was investigated. The fungus was found to grow best in barley grain mixed with
Murashige and Skoog solution over 30 days following inoculation. Further incubation at
30°C for 60 days in the dark caused numerous agglomerations of mycelia as if fruiting
bodies were being formed. The data presented provide growth requirements that will be
useful in a future development of P. portentosus as a cultivatable mycorrhizal mushroom.