From the results shown in Fig. 2., it is obvious that the two
types of IFdBSA conjugates exist and are positioned at over
200 kDa(laneIV),the one with a1:1ratio (i.e., one fluorescent
dBSA peroneantibody)that presents over half a portion of the
total number of conjugates and the other is 1:2 ratio(two
fluorescent dBSAperoneantibody).Even though a large portion
of the free antibody (anti-L. monocytogenes antibody wasusedin
this analysis)and small fluorescent dBSA still exists, approximate
efficiency of this conjugate is around 40%.It can also be noted that
most of the native BSA in lane II are labeled with a large number of
sulfhydryl reactive fluorophores after denaturation, with increasing molecular weight as shown in laneIV. The IFdBSA conjugate
characterized by its molecular state by electrophoresis was used as
a probe for fluorometric detection of bacteria in combination with
the immuno-magneticbead.