The samples were then centrifuged at 13,500 g for 10 min. After centrifugation, 500 ml of the supernatant was mixed with 1 ml of Wizard resin (Promega, Madison, USA) and pushed through a Wizard minicolumn (Promega, Madison, USA).
The samples were then centrifuged at 13,500 g for10 min. After centrifugation, 500 ml of the supernatant was mixedwith 1 ml of Wizard resin (Promega, Madison, USA) and pushedthrough a Wizard minicolumn (Promega, Madison, USA).