Agave syrups are gaining popularity as new natural sweeteners. Identification, classification and
discrimination by infrared spectroscopy coupled to chemometrics (NIR-MIR-SIMCA-PCA) and HPAECPAD
of agave syrups from natural sweeteners were achieved. MIR-SIMCA-PCA allowed us to classify
the natural sweeteners according to their natural source. Natural syrups exhibited differences in the
MIR spectra region 1500–900 cm1. The agave syrups displayed strong absorption in the MIR spectra
region 1061–1063 cm1, in agreement with their high fructose content. Additionally, MIR-SIMCA-PCA
allowed us to differentiate among syrups from different Agave species (Agave tequilana and Agave
salmiana). Thin-layer chromatography and HPAEC-PAD revealed glucose, fructose, and sucrose as the
principal carbohydrates in all of the syrups. Oligosaccharide profiles showed that A. tequilana syrups
are mainly composed of fructose (>60%) and fructooligosaccharides, while A. salmiana syrups contain
more sucrose (28–32%). We conclude that MIR-SIMCA-PCA and HPAEC-PAD can be used to unequivocally
identify and classified agave syrups.