EEO of ketorolac also
reverted on the same column when ACN and EtOH were
used as alternative mobile phases (Fig. 6c). The mobile
phase may interact with a particular chiral selector, chiral
analyte, or both and also may affect enantioselective (and
also non-enantioselective) selector–selectand interactions
without entering in point-by-point interactions with any
of the counterparts. Alternatively, the medium may affect
the conformation of the chiral selector [44, 45]. Thus, it
is interesting to learn which of above-mentioned effects is
responsible for the reversal in affinity observed due to the
change in mobile phase in this study.