This protocol was described as an efficient method for removing polyphenols, polysaccharides,
and RNA. It utilizes SDS as a detergent, PVP for binding the phenolic compounds, proteinase
K for removing proteins, potassium acetate and isopropanol for precipitation, a high-molar
concentration of sodium chloride for inhibiting coprecipitation of polysaccharides and DNA,
and an improved method for removing RNA by selective precipitation with lithium chloride.
This protocol was described as an efficient method for removing polyphenols, polysaccharides,and RNA. It utilizes SDS as a detergent, PVP for binding the phenolic compounds, proteinaseK for removing proteins, potassium acetate and isopropanol for precipitation, a high-molarconcentration of sodium chloride for inhibiting coprecipitation of polysaccharides and DNA,and an improved method for removing RNA by selective precipitation with lithium chloride.
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