Helium was used as the carrier gas at a flow rate of 30 cm/s.
The initial temperature of the column was set at 170 C and increased to 225 C with a rate of 1 C/min and then held at 220 C for an additional 20 min.
The detector temperature was set at 270 C,while the temperature at the injection port was maintained at
250 C.
Retention time of FAME standards was used to identify chromatographic peaks.
Peak area was quantitated and expressed as % of total saponifiable lipids (AOAC, 2000).