This work evaluated the use of a culture enriched in DMRB as a strategy to enrich ARB on anodes. DMRB
were enriched with Fe(III) as final electron acceptor and then transferred to a potentiostatically-controlled
system with an anode as sole final electron acceptor. Three successive iron-enrichment cultures were
carried out. The first step of enrichment revealed a successful selection of the high current-producing
ARB Geoalkalibacter subterraneus. After few successive enrichment steps, the microbial community
analysis in electroactive biofilms showed a significant divergence with an impact on the biofilm
electroactivity. Enrichment of ARB in electroactive biofilms through the pre-selection of DMRB should
therefore be carefully considered.