From the resulting mixtures, 10-lL aliquots were mixed with
1 lL of loading buffer for DNA and loaded into the sample wells
of 1% (w/v) agarose (agarose type I-A, from Sigma–Aldrich) gel,
prepared with tris–EDTA buffer (TAE) 1% (v/v) (Sigma–Aldrich)
and 10 lL of ethidium bromide 10 mg/mL (Sigma–Aldrich).