2.10. Statistical analysis
All data were analyzed using the Statistical Analysis System
(SAS/STAT version 8.2, SAS Institute Inc., Cary, NC, USA) PC package,and are presented as the mean±SD or±SEM. Statistically significant differences (p< 0.05) in replica analysis and biomarkers of skin wrinkling between the control and experimental groups were identified by Student’s t-test. All other differences were evaluated using Duncan’s multiple-range test. Statistical significance was assigned at p< 0.05.