analytical response of each ARV, including pH, surfactant concentration,
equilibration time and temperature, were study and optimized;
as well as its coupling to UFLC-ESI-MS/MS. The analytical
performance of the proposed method was evaluated in terms of
quantification limits (LOQ), repeatability, reproducibility, accuracy,
recovery and linear working range. Moreover, the methodology
was applied for the determination of ABC, EFV, 3 TC and NFV in
human plasma samples of patients under treatment and its
robustness was evaluated in terms of recovery factors (RF%).