c) Add D-larvae to the vessel at the appropriate density.
d) Calculate, following the procedure given in part 5.2.3.2, The volumes of harvested algae to add to the vessels to provide the required food ration.
e) Turn on the flow of air so that there is a good turnover of water to suspend and evenly mix both the larvae and food.
f) The culture is then left for 24 hours before further husbandry is necessary.