In order to formulate a more synergistic and effective propagation strategy for this medicinally important endangered orchid taxa which is facing threats of extinction due to anthropogenic pressures and habitat destruction, we describe here the efficiency of TDZ on high frequency micropropagation of D. nobile from pseudostem segments. Genetic variability within the propagated plants was also analyzed using RAPD and SCoT markers to determine the efficacy of the protocol from the conservation aspects. Biochemical parameters and their yields viz. total phenolic content, total flavonoid content and antioxidant activity were investigated and compared between the mother and TDZ induced micropropagated plants.