In procedure (II), in order to make the process viable using a reduced volume of reagent in the dye formation step, the reaction was carried out using a transparent 96-well plate, with 300 µL volumes of solution in each well. A 200 μL aliquot of sample solution was transferred to each well, followed by addition of 100 μL of GS reagent. After allowing to rest for 20 min, the plate was placed on the scanner and the digital image was acquired