High glucose level resulted in stronger growth impairment than moderate ethanol content. S. cerevisiae strains experienced a moderate growth inhibition by osmotic stress as compared to the control condition ( Fig. S3). The effect of this stress factor was also moderate for several other yeast strains, which reached OD600 values similar or higher than the control after 24 to 48 h of incubation. Growth of some strains to higher OD600 levels under glucose stress is due to the higher availability of glucose as carbon source. Strains of S. stipitis, C. sake and P. membranifaciens, were especially sensitive to osmotic stress by glucose, whilst S. bombicola and Debaryomyces strains showed an intermediate sensitivity.