Splenocytes were isolated from their spleens under the sterile conditions by straining through stainless sieve and cotton gauze and centrifugation on Histopaque 1077 (Sigma-Aldrich, USA) for 8 min at 400 g in order to remove erythrocytes
Splenocyteswere isolated from their spleens under the sterile conditionsby straining through stainless sieve and cotton gauzeand centrifugation on Histopaque 1077 (Sigma-Aldrich,USA) for 8 min at 400 g in order to remove erythrocytes