EGF is also responsible for increasing the diameter of follicles cultured in vitro for several species.
For bovine follicles, a study by Wandji et al. (1996) demonstrated that after 6 days of culture, the addition of 50 ng/
mL EGF to the medium promoted the maintenance of follicular viability and increased the diameter and the production of estradiol and progesterone in primary and secondary follicles (60–179 lm) isolated from fetal ovaries.