2.2.7. Effect on Liver and Kidney Functions
Mature rats of 150-180 gm were divided into 3 equal groups. The 1st group was left as a
control, while the 2nd and 3rd were orally given the plant extract in doses of 50 and 100 mg/kg b.wt. for
21 days. Blood samples were collected from the orbital plexus of each rat and sera were separated.
The sera were used to determine the activity of alanine aminotransferase (ALT) and aspartate
aminotransferase (AST) [16]. Levels of total proteins [17], albumine [18], globulin, urea [19] and
creatinine were estimated [20].
3. Results and Discussion
3.1. Phytochemical studies
3.1.1. Identification of Phenolic acids by HPLC
The identified phenolic compounds were phenol, protocatechuic acid, p-hydroxy benzoic acid,
gallic acid, p-coumaric acid and o-coumaric acid. 10 unknown compounds are also detected as shown
in Table (1).