About 10 g of MCC were mixed with 200 mL sulfuric acid aqueous
solution (64 wt.%) in a three-neck flask, which were set with
stirrer, thermometer and condenser, respectively. The reactive
mixture was kept stirring for 1 h at 45 ◦C for hydrolysis, then about
200 mL of cold water (about 0 ◦C)was poured into the obtained suspension
to stop the reaction. Subsequently, the suspension was
centrifuged at 10,000 rpm till it had no obvious stratification and
existed as a kind of transparent dispersion. After that, dialysis
against distilled and deionized water was kept going for several
days till the pH of the water reached a value of 7.0, in order to
remove the remained free acid in the dispersion. Finally, cellulose
nanocrystal (CNC) was received after freeze-drying.
About 10 g of MCC were mixed with 200 mL sulfuric acid aqueoussolution (64 wt.%) in a three-neck flask, which were set withstirrer, thermometer and condenser, respectively. The reactivemixture was kept stirring for 1 h at 45 ◦C for hydrolysis, then about200 mL of cold water (about 0 ◦C)was poured into the obtained suspensionto stop the reaction. Subsequently, the suspension wascentrifuged at 10,000 rpm till it had no obvious stratification andexisted as a kind of transparent dispersion. After that, dialysisagainst distilled and deionized water was kept going for severaldays till the pH of the water reached a value of 7.0, in order toremove the remained free acid in the dispersion. Finally, cellulosenanocrystal (CNC) was received after freeze-drying.
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