Fungal isolation and identification
Aspergillus isolates were obtained from maize and other food
sources following surface sterilization in 70% alcohol and plating on
Potato Dextrose Agar (PDA) medium. Pure culture of each isolate
was obtained after incubation at room temperature. Identification
was effected by mounting fungal mycelium on lactophenol-cotton
blue and observing under x 40 objective lens of the microscope.
Colony colour, growth pattern on plates, details of philiades and
spores were also used as identification parameters with reference
to Raper and Fennel (1965).