Therefore, this study explored the possibility of using enucleated pig oocyte as a universal recipient cytoplast for production of transgenic cloned embryos by iSCNT. Donor cells, stably expressing the enhanced green fluorescent protein (EGFP) gene, from phylogenically distant cattle, mice, and chicken were used to test this possibility. We further investigated if xenogenic mitochondrial transfer from somatic cells to recipient cytoplast was a cause for the reduced embryonic development of iSCNT embryos.