In the second part of this research, addressed to the study of
expression of the THCA synthase gene by reverse-transcriptase RT-
PCR, the RNA of 12 cannabis samples (from CS7 to CE18) was
investigated using the Rubisco gene and the 26S ribosomal RNAgene
as internal control genes for constant expression. The results did not
show a definite correlation between the chemical concentration of
THC and THCA synthase gene expression, even if the samples with
the highest percentage of THC also showed increased relative
expression; on the other hand, for similar classes of Cannabis
samples, the expression of the THCA synthase have shown a
correlation with the analysed part and with the maturity of the plant.