In conclusion, we for the first time developed a stable nuclear transformation
method and a feasible fluorescent reporter system for
C. vulgaris, an industrially important microalga with great potential for
CO2 biomitigation, which on the one hand provides a solid foundation
for the future rational genetic manipulation for strain improvement
such as CO2 fixation capacity, and on the other hand will allow great applications
in cell biology for protein subcellular localization by EGFP
fusion