however multiplied at a slower rate than the acid formers and were found to be very sensitive to environmental changes, such as pH (Table 4). Fungal isolates include Aspergillius, Rhizopus, Penicillium and Mucor; their major source was the human excreta. Pritchard et al. (2009) reported a similar result when they isolated Escherichia coli, Aspergillius, Clostridium botulinum, Clostridium chavoie from water contaminated by human excreta in Malawi.