Determination of endogenous hydrogen gas
(H2) content
For analyzing endogenous H2 content, headspace sampling
of gas followed by gas chromatography (GC),
which was previously used to determine endogenous
H2 and carbon monoxide (CO) contents in animal
tissues (Renwick et al. 1964; Bernardi et al. 2008),
was adopted with minor modifications according to
the method described in our previous reports (Jin et al.
2012; Xie et al. 2012)