culture
Shoots from the first rose genotype were collected
on October 15, 2009 from the experimental field Pohorski
dvor, which belongs to the Faculty of Agriculture and Life
Sciences, Hoče, NE Slovenia. Shoots of the second and the
third genotype were collected in Tepanje (a village near
Maribor, NE Slovenia) on May 26, 2010. In the laboratory
for tissue culture, nodal explants taken from newly formed
shoots were dissected with a scalpel and used as a source for
explants. Prior to surface sterilisation, shoots were cut into
two-node explants about 15 mm in length, leaf blades were
removed leaving only a small basal section of petiole (Fig. 1).