Fecal and urine collectionsคอลเลกชันอุจจาระและปัสสาวะ
Tatal 24 h collection of feces and urine was performed immediately following morning diet allocations on each of the 4 collection days
Thirty mL of 6 N HCL was added to every urine collection pail at the start of each collection day after the previon day's
collection was complete
Urine was weighed and volumes recorded;100 mL subsamples were frozen and stored at -20 C until later analysis
Collection of feces was initiated immediately following the morning feed allotment and ended 96 h later
Collected feces were weighed and stored at -20 C
At the end of each experimental period, total collected feces were pooled within barrow and remained frozen until later
analysis
in a forced air oven (Model number LHD 2-14, Despatch Oven Co., Minneapolis, MN, USA) at 65 C for 48 h
Fecal dry matter was determined by first, thawing and mixing pooled feces within barrow then, drying a 1 kg subsample