Preparation of Ethanol Extracts of A. paniculata. Simplicia A. paniculata leaves were oven-dried, then grinded with a grinder until becomes a fine powder. Furthermore, the powders was extracted by maceration with ethanol 90% and stirred for 24 h. The macerate then filtered with a vacuum pump and evaporated with a rotary evaporator at 40 °C to obtain a viscous extract. The ethanol extract then analyzed using High Performance Liquid Chromatography (HPLC) to determine the content of active compounds andrographolide.