Endophytes are diverse and often grow readily in culture. With recent technological advances, the use of culture-free methods promises to discover ever-greater diversity and to expand our understanding of the structure of the fungal tree of life. One particularly useful aspect of culture-free methods may be to show that particular fungi are present in an environment, thus leading endophyte researchers to optimize culturing conditions as a means to capture those fungi in vitro. Such efforts are critical for establishing voucher specimens, which in turn can be used to empirically assess species interactions, as raw materials for bioprospecting or biological control, and as the basis for future research in systematics or genomics. Because many endophytes do not sporulate in culture, and thus are classified only as mycelia sterilia, they are not welcome at most established culture collections. Depositories are needed to house and maintain vouchers of these fungi, and to curate their ecological data (site of origin; host plant; season; tissue type). Both the specimens themselves and the data regarding their recovery have tremendous intrinsic value, (Arnold 2007).