Abstract: A regeneration protocol was developed using cotyledonary node explant on MS medium
supplemented with different concentrations of cytokinins viz; BA (6-benzylaminopurine), Kn (kinetin) and TDZ
(thidiazuron) individually or in combinations. BA (2.5 mg L ) 1 and Kn (1.0 mg L 1) induced maximum numbers
of multiple shoots with 80% response. Proliferation and elongation were achieved on the same medium
containing lower concentrations of cytokinins BA + Kn (1.5 + 1.0 mg L 1), elongated shoots were rooted on
half strength MS medium containing IBA 5.0 mg L 1 produced maximum number of roots with 60% response.
The plantlets were gradually acclimatized and successfully transferred to field condition with 60% survival rate
was recorded.