1. Perform the antimicrobial susceptibility testing in a biological safety cabinet (BSC).
2. Prepare inoculum of B. pseudomallei isolated colonies selected from an 18-24 h non-selective agar plate to get a concentration of approximately 1x10 8 CFU/mL (usually equivalent to a 0.5 McFarland standard).
3. Apply the bacterial inoculum onto the surface of Mueller Hinton agar.
4. Dispense preferred set of antimicrobial disks (e.g. CAZ, MEM, IMP, AMC, DO and SXT).
5. incubate plates at 35ºC for 18-24 h.
6. The resulting zones of inhibition need to be uniformly circular with confluent lawn of growth.