The hydrophobicity of peptides from TRMHs was studied by
reversed phase HPLC using an Agilent 1100 HPLC system(Agilent Technologies,
CA, USA). The column used in this experiment was a Symmetry
C18 (4.6 × 250 mm, 5 μm) from Waters Co. (Milford, MA, USA).
Solvent A was TFA in bidistilled water (0.1% v/v) and solvent B
contained TFA (0.085% v/v) in acetonitrile (ACN):bidistilled water
(60:40 v/v). Both mobile phases A and B were filtered through a
0.45 μm nylon membrane filter and degassed prior to any analytical
run. Peptides were first eluted with 100% solvent A for 2 min, followed
by a linear gradient from 0% to 50% of solvent B during 50 min at a flow
rate of 1 ml/min. The separation was monitored at a wavelength of
214 nm.