A fungal strain was isolated from papaya with anthracnose
symptoms and identified as C. gloeosporioides Penz. The phytopathogen
was maintained on Potato Dextrose Agar (PDA; Difco)
at 4 ◦C. Conidial suspensions (1.0
×
105 conidia/mL) were prepared
from new cultures incubated for 10 d at 28 ◦C. The cells were
counted using a Neubauer chamber under optical microscope
(Nikon E-200) and concentration adjusted to 1.0
×
108 cell/mL
(Zhao et al., 2008).