This work reported the purification through acetone and ATPSs and the characterization
of PPO from the Cape gooseberry fruit for the first time. The PPO activity decreased as
the fruit matured and ripened. The enzyme was more active and specific toward
chlorogenic acid substrates at optimal pH and temperature values of 5.0 and 20 ºC,
respectively. The PPO activity was strongly inactivated by quercetin, ascorbic acid and
L-cysteine. This study shows possible treatments that can be implemented during the
processing of Cape gooseberry fruits to prevent the browning effect and to allow for the
use of cracked fruits. However, further research is needed to determine enzyme
characteristics and propose more effective treatments applicable at the industrial scale.
Acknowledgements
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This work was supported by the Committee for the Development of Research (CODI)
of the University of Antioquia (SIU-341373).