The upper layer (2.5 mL) was mixed with distilled water (2.5 mL) and
freshly prepared 0.1% ferric chloride solution (0.5 mL). Absorbance
was measured at 700 nm. After subtracting absorbance of a blank
sample (prepared from distilled water), FRP was determined from
ascorbic acid (AA) calibration standard as AA equivalents in milligram per kilogram of carrot juice or yoghurt