2. Materials and methods
2.1. Experimental design
Twenty glass tanks (90 cm 40 cm 35 cm) filled with 100 L
seawater were used as the experimental culture units. Temperature
in all tanks was maintained in the range of 27.3e28.3 C during the
entire experiment, aeration was provided in each aquarium using
an air blower and the light regime was set at 12 h light/12 h dark.
Inter-molt phase Pacific white shrimp juveniles, previously acclimatized
collectively to the experimental room and conditions for 1
week, at an initial average body weight of 2.02 ± 0.05 g were
randomly distributed in the tanks at a density of 30 shrimp/tank
(83 shrimp m2). Four times daily, a commercial pellet containing
30% of crude protein (Feng Li, PT Matahari Sakti, Indonesia) was
provided for 49 days to all tanks. The feeding level was determined
at 7% on wet body weight per day and the daily feed amount was
adjusted to the biomass in the tanks.
2. Materials and methods
2.1. Experimental design
Twenty glass tanks (90 cm 40 cm 35 cm) filled with 100 L
seawater were used as the experimental culture units. Temperature
in all tanks was maintained in the range of 27.3e28.3 C during the
entire experiment, aeration was provided in each aquarium using
an air blower and the light regime was set at 12 h light/12 h dark.
Inter-molt phase Pacific white shrimp juveniles, previously acclimatized
collectively to the experimental room and conditions for 1
week, at an initial average body weight of 2.02 ± 0.05 g were
randomly distributed in the tanks at a density of 30 shrimp/tank
(83 shrimp m2). Four times daily, a commercial pellet containing
30% of crude protein (Feng Li, PT Matahari Sakti, Indonesia) was
provided for 49 days to all tanks. The feeding level was determined
at 7% on wet body weight per day and the daily feed amount was
adjusted to the biomass in the tanks.
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