2.5. Inoculation of rice wine with the tested pathogens and
B. cereus spores
To obtain samples containing approximately 3e4 log CFU or
spores/ml, appropriate dilutions of the three-strain mixtures or
B. cereus spore suspensions were inoculated into turbid rice wine
and mixed by gentle shaking to ensure uniform distribution. Portions
(10 ml) of each fermented alcoholic beverage were dispensed
into individual sterile glass tubes. Twenty tubes containing sterilized
or non-sterilized turbid rice wine were used for each bacterial
sample or spore sample; the pH and survival rates [at 1, 3, 7, 14, and