differential and selective medium.
A volume of 50 mL of semen were incubated in 10 mL of
BHI broth (OXOID) at 37 C. After 24 hours, more than onethird
of them showed turbidity, which is indicative of
microorganism growth. Then, the bacterial cultures were
centrifuged at 5000 g for 10 minutes and submitted to
MS analysis, described as follows. For the isolation of single
colonies, decimal dilutions of the bacteria pellet were
plated on BHI/agar (2% wt/vol malt extract agardOXOID)