As the same for SSF3 process, both alpha-amylase (Spezyme Alpha) at 3,532 AAU/kg CF and beta-glucanse (Optimash TBG) at 2,812 U/kg CF were added for liquefaction, For SSF, two glucoamylases in Distillase ASP at 540 GAU/kg CF and in Amigase Mega L at 0.035% w/w were added to the liquefied mash to improve the efficacy of hydrolyzing residual starch.