The analysis of variance (ANOVA) and surface plots were generated
using Design Expert 7.1, and the optimized value of four independent
variables for best response was determined using a
numerical optimization package of the same software. An experiment
was also planned using enzyme concentration, hydrolysis
temperature and time generated by the Design Expert software
(Table 1), but without addition of yeast inoculum to establish the
role of enzymatic hydrolysis in production of sugars from the insoluble
polysaccharide fractions