Inoculums for soybean fermentation was prepared by inoculating
20 g of cooked and dehulled soybeans with overnight
grown cultures of B. subtilis (MTCC 5480 and MTCC 1747) followed
by incubation at 42 °C for 24 h.The fermented inoculum
had count of 8.48 and 8.18 log (cfu)/g of soybean for B. subtilis
MTCC 1747 and B. subtilis MTCC 5480, respectively. For SSF of
cooked soybean, 200 g of cooked and dehulled soybeans were
inoculated with 10 g of the 24 h fermented starter and incubated
at 42 °C for 24 h (Fig. 1). After fermentation, soybeans were
dried at 60 °C and powdered using mixer grinder to obtain fine
powder (particle size < 200 microns) for further analysis.