3.5. Antimicrobial activity using microtiter plates
Based on the results of simple well diffusion assay (Vinderola
et al.,2008), the LAB isolates that showed inhibitory activity
against non pathogenic E. coli K12 (datanotshown) were
further confirmed by microdilution technique using 96 well
microtiter plates. During antimicrobial assay in microtiter
plates, the growth of each pathogenic bacterium in serially
diluted CFS(1:1,1:10and1:100)was compared with their
growth to control BHI broth (withoutCFS) medium and the
antimicrobial activity was measured as% growth inhibition.
As it can be seenin Fig. 1a and b, in the1:1 diluted CFS,all
LAB strains exhibited on an average 80%–90% growth reduc-
tion compared to their growth in normal BHI broth except the