To prove the correctness of the performance of the two lacZ
and uidA genes, at first standard strains and then reference
strain (CRM) were studied and the occurrence of bands in
electrophoresis was confirmed. The MPN was used as the gold
standard to minimize any error in interpretation of results. To
further confirm the results, bacteria isolated in MPN test were
identified up to the extent of species and then purified and
identified bacteria were successfully undergone molecular
evaluation with the help of these two genes. Given that the
entire study was planned with the aim of making routine tests
in order to perform operation in the Iran’s water microbiology
laboratories, the results of this study prove the operability of the usage of molecular methods in routine applications for the
microbial assessment of drinking water.