2. Materials and methods 2.1. Chemicals and reagents Methanol, acetic acid, and ethanol were of analytical grade from Merck (Australia) and all the other reagents were purchased from Sigma–Aldrich (St. Louis, USA) unless stated otherwise.
2. Materials and methods2.1. Chemicals and reagentsMethanol, acetic acid, and ethanol were of analytical grade from Merck (Australia) and all the other reagents were purchased from Sigma–Aldrich (St. Louis, USA)unless stated otherwise.