PCR was run under the following program: an initial 5 min at 95 ◦C, followed by 35 cycles of 30 s at 94 ◦C, 30 s at 55 ◦C, 30 s at 72 ◦C, and a final extension of 10 min at 72 ◦C. PCR products
were analyzed on polyacrylamide gel stained with silver nitrate