The growth of algae in treated wastewater showed a good response despite of low N and P concentration with efficient removal of COD (98.8%). Zhou and researchers [35] removed 82.27–96.18% TOC from concentrated municipal wastewater, whereas Chinnasamy et al. [3] MANAGED to remove >96% nutrients from carpet mill effluents. The nutrient consumption profile confirmed that PO4–P, NO3–N and NH4–N in the culture media was depleted in 4 days, and reached up to 98.8% and 99.4%, respectively, whereas NH4–N was found zero even after a short period of 18 h of culture (Table 4). Since the first day of culture the biomass and chlorophyll a increased with time but, after 4 days of incubation unexpectedly the biomass showed increasing trend although determination of chlorophyll a did not show any significant changes during culture (data not shown). However, the nitrogen in the medium was depleted within 4 days of incubation. Hecky and Kilham [16] and Chinnasamy et al. [3] had also noticed the same trends. The above observations clearly proved that growth in biomass after 4 days of incubation was due to increase in internal cellular nutrients rather than increase in number of algal cell. In nitrogen limiting medium, the carbon fixation during photosynthesis preferred synthesis of carbohydrate rather than to opt the path of protein synthesis [12] and [29].