Twenty-three cement ponds in a private hatchery fish in Kafr El Sheikh Governorate with dimensions of 3x 8x 2 meter and Twelve glasses aquaria with dimension of 70 x 53 x 53 cm. in Labe of Hydrobiology Dept. NRC, were used for the biological
and innocuty test Cement ponds and glasses aquaria were supplied with dechlorinated water with a temperature(25:28 Co).
Diet: diet with 35% and 25% protein for feeding of fingerlings and adult Nile tilapia respectively. Food in ratio of 3% of fish body weight per day was considered to be the optimal maintenance amount required for adult fish and 5% for fingerlings according Noor El Deen (2OO7)