The contents of major phenylethanoid and iridoid glycosides in the calli were determined by high performance liquid chromatography(HPLC), Hampagoside, harpagide verbascoside and isoverbascoside were identified and the abil- ity to produce these bioactive compounds sustained for over 3 years, H procumbens calli were also analyzed by colorimetric methods for total contents of phenolics, flavonoids and anthocyanins, The extracts of cNB callus had the highest antiradical activity against DPPH and ABTS and capacity to reduce metal ions(Ferric Reducing Antioxidant Power-FRAP) The same extracts were also characterized by the highest total content of phenolics and flavonoids.