The samples were kept in paper bags and stored in portable
refrigerators during transfer to the laboratory for isolation of
yeasts. From each vineyard ten different bunches were randomly
selected and from each bunch ten berries were collected and transferred
in sterile distilled water (SDW) containing 0.02% Tween-20.
The berries were shaken at room temperature for 10 min at
150 rpm and then 10-fold dilutions were prepared in SDW. From
each dilution 200 ll were plated in Petri dishes containing yeast
malt agar (YMA, containing 3 g yeast extract, 3 g malt extract, 5 g
bactopeptone, 10 g glucose, 15 g agar per lt). Petri dishes were